Theoretical considerations for oocyte cryopreservation by freezing

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Theoretical considerations for oocyte cryopreservation by freezing Introduction

Attempts to cryopreserve oocytes by freezing have, to date, been based mostly on empirical approaches rather than on basic principles, and perhaps in part for this reason have not been very successful. Theoretical considerations suggest some fairly ‘heretical’ conclusions. The concentrations of permeating cryoprotectants employed in past studies have probably been inadequate, and the choice of ...

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OBJECTIVE To review historical and contemporary advances in oocyte-cryopreservation techniques and outcomes. DESIGN Publications related to oocyte cryopreservation were identified through MEDLINE and other bibliographic databases. CONCLUSION(S) Oocyte cryopreservation can be used as an adjunct to conventional IVF and as an option for fertile women to electively cryopreserve their gametes. R...

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Evaluation of the Fast Freezing Method in Cryopreservation of Human Ovarian Tissue for Oocyte Viability

The evaluation of the fast freezing method as an option for fertility maintenance, after long-term human ovarian tissue cryopreservation. Samples were obtained from premenopausal women, who have had children, who were undergoing gynecologic surgery. The samples were cryopreserved for six years. Subsequently, were thawed and examined for the presence of oocytes and were immunohistochemically eva...

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Human oocyte cryopreservation.

The clinical role of oocyte cryopreservation in assisted reproduction, as an adjunct to sperm and embryo cryopreservation, has been comparatively slow to evolve as a consequence of theoretical concerns related to efficacy and safety. Basic biological studies in the 1990's alleviated many of these concerns leading to more widespread adoption of the technology. While a number of babies were born ...

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A slow freezing/rapid thawing method for the cryopreservation of human oocytes has been employed using a sodium-depleted culture media. In 53 frozen egg-embryo transfer (FEET) cycles, a 60.4% survival rate post-thaw was obtained and a 62.0% fertilization rate following intracytoplasmic sperm injection. Overall pregnancy rates were 26.4% per thaw attempt, 30.4% per patient, and 32.6% per embryo ...

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ژورنال

عنوان ژورنال: Reproductive BioMedicine Online

سال: 2007

ISSN: 1472-6483

DOI: 10.1016/s1472-6483(10)60672-4